PCR-spezifische Puffer

Thermo Scientific™ Phusion Buffers

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases. Phusion HF Buffer Pack FP=2 x 1.5 ml

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility GlycoBlue (15 mg/ml) (0.3 ml tube), 5 tubes

Invitrogen™ UltraPure BSA (50mg/mL)

Excellent PCR stabilizer UltraPure BSA (50 mg/ml) (50 mg tube), 5 tubes

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Speicherplatzbedarf: Store at room temperature. Nuclease-free Water (not DEPC-treated) (1x 1000ml)

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR Grade Water (10 x 1.5 ml)

Invitrogen™ tYeast RNA (10mg/mL)

Co-precipitant to aid in nucleic acid recovery Yeast tRNA (10 mg/ml), 500 µl

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 1 M Tris, pH 8.0, 500 ml

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration Molecular biology grade, Ambion® PBS 10X, pH 7.4solution, 1000 ml

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 0.5 M EDTA, pH 8.0, 1000 ml

Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)

Blocks non-specific hybridization Salmon Sperm DNA (sheared, 10 mg/ml) (1 ml tube),10 tubes

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Proteinase K Solution (20mg/mL)

General digestion of proteins Proteinase K Solution (20 mg/ml) (1.25 ml tube),5 tubes

Invitrogen™ Ammonium Acetate (5M)

Ready-to-use, certified RNase-free 5 M Ammonium Acetate, 500 ml

Invitrogen™ UltraPure™ RNase A (20mg/mL)

Isolates RNA-free DNA RNASE A,10 ML

Invitrogen™ Elongase™ Enzyme Mix

Amplifies both long and short DNA templates Elongase Enzyme Mix, 100 reactions

Invitrogen™ MgCl2 (1M)

Ready-to-use, certified RNase-free Molecular biology grade, Ambion® 1 MMgCl2solution, 100 ml

Invitrogen™ Platinum™ PCR SuperMix High Fidelity

Built-in hot start PLATINUM PCR SUPERMIX HIGH FID, 5000 REACTIONS

Invitrogen™ Formamide (Deionized)

Nuclease-free, deionized FORMAMIDE (DEIONIZED) 5 X 500G

Invitrogen™ DEPC-Treated Water

Speicherplatzbedarf: Store at room temperature. DEPC-treated Water, 500 ml

Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments AccuPrime Pfx SuperMix, 200 reactions

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions Acid-Phenol:Chloroform, pH 4.5, 400 ml(with IAA, 125:24:1)

Invitrogen™ Sodium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free Ambion® 3 M Sodium Acetate solution, Molecularbiology grade, pH 5.5, 100 ml

Invitrogen™ Platinum™ Pfx DNA Polymerase

Built-in hot start Platinum Pfx DNA Polymerase 500 reactions 95514,52806

Invitrogen™ LiCl Precipitation Solution (7.5M)

Will not precipitate DNA, protein, or carbohydrates 7.5 M LiCl Precipitation sol., 100ml

Invitrogen™ TE, pH 8.0

Ready-to-use, certified RNase-free TE buffer, pH 8.0, 1000 ml

Thermo Scientific™ MgCl2 (Magnesium Chloride)

Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution. 5ML 25MM MGCL2

Invitrogen™ NaCl (5M)

Ready-to-use, certified RNase-free Natrium Chlorid 5 M, 500 ml

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase AccuPrime Pfx DNA Polymerase, 1000 reactions

Invitrogen™ PCRX Enhancer System

Optimized buffer and cosolvent system to simplify PCR amplification PCRx Enhancer System, 250 reactions

Invitrogen™ Linear Acrylamide (5 mg/mL)

Useful for separating reaction products from unincorporated nucleotides and oligonucleotide primers Linear Acrylamide (5 mg/ml) (1 ml tube), 5 tubes

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