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ACROBiosystems Human PH20 / SPAM1 Protein, His Tag (active enzyme)

Marke:  ACROBiosystems PH0-H5225-25ug

Weitere Details : Netto-Gewicht : 0.00001kg

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Artikelnummer. 15825345

  • 383.89 € / 25 Mikrogramm
Verfügbar ab: 09-04-2024
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Beschreibung

Beschreibung

Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Spezifikation

Spezifikation

NP_694859.1
25 μg
Leu 36 - Tyr 482
SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15
Unconjugated
His Tag
Less than 1.0 EU / μg by the LAL method.
Powder
>95% as determined by SDS-PAGE
53.0 kDa
Human PH20, His Tag (PH0-H5225) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Tyr 482 (Accession # P38567-1).
-20°C
PH20
Human
50 mM Tris, 100 mM NaCl, pH7.5
HEK293
Enzymes
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Zertifikate
Sonderangebote

Sonderangebote