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ACROBiosystems Recombinant Protein L Protein, His Tag
Recombinant Protein
Marke: ACROBiosystems RPL-P3141-2MG
Weitere Details : Netto-Gewicht : 0.00001kg
Beschreibung
Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
Recombinant Protein L, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 45 kDa polypeptide. Protein L Protein can bind to VL-Kappa.Spezifikation
| 2 mg | |
| -20°C | |
| Protein L | |
| His Tag | |
| Less than 1.0 EU / μg by the LAL method. | |
| Powder | |
| >95% as determined by SDS-PAGE |
| Recombinant Protein L, His Tag, also known as Recombinant Peptostreptococcus magnus Protein L, was expressed in E.Coli at ACRObiosystems. | |
| RPL,Protein L | |
| Unconjugated | |
| 100 mM NaH2PO4, pH8.5 | |
| E.coli | |
| MABSol™ Affinity Media Ligands |