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Thermo Scientific™ ABsolute™ qPCR Master Mixes für probenbasierten Nachweis
Die qPCR-Mastermixe Thermo Scientific™ ABsolute bieten Sensibilität und Reproduzierbarkeit für Echtzeit-PCR-Anwendungen.
N.V.
Spezifikation
| Inhalt und Lagerung | ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated). Store at -20°C. Avoid freeze/thaw cycles |
|---|---|
| Aktivierungszeit | heated to 95°C for 15 minutes |
Beschreibung
Genießen Sie Empfindlichkeit und Reproduzierbarkeit in Echtzeit-PCR-Anwendungen mit den Thermo Scientific™ Absolute™ qPCR-Mastermischungen, deren Nachweis auf Proben basiert.
ABsolute qPCR Master Mixes are designed to achieve the most consistent and reproducible data across all QPCR platforms. Proprietary additives give enhanced and consistent end point readings as well as low Ct values. All mixes contain Thermo-Start Taq DNA Polymerase, for increased specificity, and dTTP for increased reaction efficiency and sensitivity (low Ct).Maximum specificity—Chemically-modified Thermo-Start™ Taq remains completely inactive until heated to 95°C for 15 minutes, thereby preventing nonspecific amplification
Improved efficiency—The use of dTTP in place of dUTP increases reaction efficiency by approximately 5% and therefore reduces reaction time
Increased sensitivity—The combination of Thermo-Start Taq and dTTP increases sensitivity and allows for detection of low copy number templates
Instrument specific—Individually optimized qPCR mixes designed for all major qPCR / Real-Time PCR instruments
Reproducible results—All mixes undergo extensive quality control testing to ensure batch-to-batch consistency
Simple protocol—Master mix format reduces protocol steps and minimizes risk of pipetting errors and sample contamination
Includes:
ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated)
Recommended for:
Ideal for qPCR analysis in molecular biology applications, including gene expression and SNP typing
Spezifikation
| ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated). Store at -20°C. Avoid freeze/thaw cycles | |
| heated to 95°C for 15 minutes |
Der Erwerb dieses Produkts umfasst eine Immunität gegenüber Rechtsschutzverfahren gemäß den in der Produkteinlage angegebenen Patenten für den ausschließlichen Gebrauch dieser Menge des Produkts für eigene interne Forschungsanwendungen des Käufers. Darüber hinaus weder keinerlei weitere Patentrechte übertragen, weder ausdrücklich noch implizit oder durch Rechtsverwirkung. Weitere Informationen zum Erwerb von Lizenzen erhalten Sie vom Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.