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anti-6x-His Epitop-Tag-Klon: HIS.H8

Mouse Monoclonal Antibody

Marke:  Invitrogen MA121315BTIN

Weitere Details : Netto-Gewicht : 0.01000kg

Artikelnummer. 15287848

  • 187.00 € / 1 Stück


Voraussichtlicher Liefertermin 30-07-2019
Zum Warenkorb hinzufügen

Beschreibung

Beschreibung

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering. By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Spezifikation

Spezifikation

6x-His Tag
Monoklonal
1 mg/mL
100 µg Protein A-aufgereinigter Antikörper (1 mg/ml) in 10 mM PBS, pH 7.2.
His Tag; Histidine Tag; Histidine Epitope Tag; 6X His eptope tag; 6His tag; 6X His
Synthetisches Peptid mit 6x His.
Protein A
RUO (Nur für Forschungszwecke)
Primär
Monoclonal
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
HIS.H8
Unmarkiert
Gereinigt
Maus
IgG2
100 µg
Kurzfristig bei 4 °C lagern. Längerfristig bei -20 °C lagern. Wiederholtes Einfrieren/Auftauen vermeiden.
Primary
Tag
Videos
Dokumentation