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beta Catenin Polyclonal Antibody, Invitrogen™
Rabbit Polyclonal Antibody
Marke: Invitrogen PA516762
Weitere Details : Netto-Gewicht : 0.01000kg
Beschreibung
This antibody reacts with the C-terminal portion of beta-Catenin. For staining of formalin-fixed, paraffin-embedded tissues, it is recommended to boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls are MCF-7 cells and breast carcinoma tissue. This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintece of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.Spezifikation
beta Catenin | |
Polyclonal | |
PBS with 1% BSA and 0.1% sodium azide; pH 7.6 | |
P26233, Q02248, Q9WU82, P35222 | |
CTNNB1, ctnnb1.L | |
A synthetic peptide from the C-terminus of the beta-catenin protein | |
500 μL | |
Primary | |
Chicken, Human, Mouse, Rat, X. laevis | |
Antibody | |
IgG |
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot | |
Unconjugated | |
CTNNB1 | |
catenin beta-1, Catenin beta, CATNB, CTNB1, CTNNB, catenin | |
Rabbit | |
Affinity chromatography | |
RUO | |
395964, 84353, 1499, 12387, 399274 | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid |