Lactoferrin Sheep anti-Canine, Human, HRP, Polyclonal, Invitrogen™

Beschreibung

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is mammary gland. Sheep anti Human lactoferrin antibody recognizes human lactoferrin (LTF), an approximately 80 kDa non-haem, iron-binding globular glycoprotein and member of the transferrin family present in milk and mucosal secretions, and also released from neutrophil granules during inflammation.

Lactoferrin (LF) is an iron binding glycoprotein. LF consists of a single polypeptide chain (approximately 80 kDa) folded into two structurally homologous lobes, each of which can reversibly bind one ferric ion (Fe3+). LF is found in external fluids, including milk and mucosal secretions, and is a prominent component of secondary granuals of neutrophils. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. LTF acts as a major first line defense against microbial infections, partly due to its ability to efficiently bind to and hence remove Fe3+ from the environment, but studies have also shown that LTF possesses bactericidal properties and proteolytic activity, capable of cleaving arginine-rich sequences within microbial proteins. Three isoforms of LTF have been identified: LTF-alpha, LTF-beta and LTF-gamma and receptors for LTF are expressed in the gastrointestinal tract, by macrophages, lymphocytes, neutrophils and platelets and also by some bacteria.