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Mouse IgM, DyLight 488, clone: PFR-03, Isotype Control, Invitrogen™
Mouse Isotype Control
Marke: Invitrogen MA1194D488
Weitere Details : Netto-Gewicht : 0.00900kg
Beschreibung
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
MA1-194-D488 can be used as a negative control in any immunoassay where a DyLight 488-conjugated primary antibody of the mouse IgM isotype is used. DyLight 488 has an excitation/emission of 493/518nm.Spezifikation
| Mouse IgM | |
| PFR-03 | |
| DyLight 488 | |
| IgM, IgM mu | |
| selective precipitation | |
| RUO | |
| Not Applicable | |
| Liquid |
| Flow Cytometry, Immunocytochemistry, Immunofluorescence | |
| 0.1mg/mL | |
| PBS with 0.2% BSA and 15mM sodium azide; pH 7.4 | |
| Mouse | |
| 100 μg | |
| Isotype Control (Primary) | |
| Store at 4°C, do not freeze | |
| IgM |