Phospho-Caveolin 2 (Tyr19) Polyclonal Antibody, Invitrogen™

Beschreibung

PA1-060 detects phospho-caveolin-2 Y19 from human and mouse samples. PA1-060 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ∽21 kDa protein representing phospho-caveolin-2 Y19 from extract from COS-7 cells transiently transfected with the mouse caveolin-2 gene. Immunofluorescent staining of phospho-caveolin-2 Y19 in NIH 3T3 cells using PA1-060 results in a distinct punctate staining pattern at the cell periphery. The PA1-060 immunogen is a synthetic phosphopeptide corresponding to residues M(14) A D D A (pY) S H H S G C(25) of mouse CAV2. This peptide (Cat. # PEP-180) is available for use in neutralization and control experiments.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly