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![BRAF [V599E] Recombinant Human Protein 10μg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-650.jpg)
BRAF [V599E] Recombinant Human Protein
BRAF [V599E] Recombinant Human Protein
Marke: Invitrogen PV3849
Weitere Details : Netto-Gewicht : 0.05250kg
Beschreibung
BRAF acts upstream of MEK in the MAP kinase pathway and is involved in cell division, differentiation, and secretion. The BRAF mutant V599E is oncogenic and found in a variety of cancers.Spezifikation
| NP_004324.1, V599E | |
| Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
| 67.1kDa | |
| 10μg | |
| For research use only. Not for use in diagnostic procedures | |
| BRAF V599E | |
| BRAF V599E was pre-diluted in enzyme dilution buffer and assayed in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100μM ATP, 200μg/mL MAP2K1, inactive (P3093) and trace [32P]-γ-ATP for 10 minutes at 30°C | |
| Amino acids 416-766 | |
| 260nmoles of phosphate transferred to MAP2K1, inactive (P3093) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.25μg/mL | |
| GST Tag | |
| Kinase and inhibitors | |
| 65% by SDS-PAGE |
| 0.27mg/mL | |
| 10488, 673 | |
| BRAF [V599E] Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| BRAF [V599E] Recombinant Human Protein | |
| RAF | |
| BRAF [V599E] Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| Serine/threonine kinase |
For Research Use Only. Not for use in diagnostic procedures.