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Invitrogen™ Canine TNF-alpha Recombinant Protein

Canine TNF-alpha Recombinant Protein

Marke:  Invitrogen™ RCTNFAI

Weitere Details : Netto-Gewicht : 0.80000kg

Artikelnummer. 11898761

  • 433.00 € / 20 Mikrogramm
Verfügbar ab: 16-04-2024
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Nur für Forschungszwecke. Die Anwendung muss den Produktanweisungen entsprechen.


Beschreibung

Beschreibung

Canine TNF-alpha Recombinant Protein for Western Blot, IP, ELISA

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C. Activity: The biological activity of recombinant canine TNFa was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D. The ED50 (or 1 unit of activity) for this effect in our laboratories is typically 10 pg/mL. Refs: Kitani, H. et al. (2002) Infect. Immun. 70:2210. Khabar, K.S. et al. (1995) Immunol. Lett. 46:107. Expression: The mature, soluble form of canine TNF-alpha was expressed in Pichia pastoris, X-33. GenBank™ Accession &Num; P51742, amino acids 77 - 233. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VKSSSRTPSD. Reconstitute with 0.5 mL of distilled water.
Spezifikation

Spezifikation

P51742
PBS with no preservative
Canine TNF alpha
Yeast
RUO
Canine TNF alpha
Canine
Ca
Recombinant
Recombinant
ELISA, Immunoprecipitation, Western Blot
403922
20μg
4°C
Canine Tumor Necrosis Factor alpha TNFA
TNF
Unconjugated
Yeast
Lyophilized
purified
Produktvorschläge

Produktvorschläge

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Dokumentation

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Sonderangebote

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