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  5. Invitrogen™ CCL4 (MIP-1 beta) Human Uncoated ELISA Kit

Invitrogen™ CCL4 (MIP-1 beta) Human Uncoated ELISA Kit

Artikelnummer. 15531007
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Artikelnummer. 15531007 Lieferant Invitrogen™ Lieferanten-Nr. 88703488

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Beschreibung

Specifically engineered for accurate and precise measurement of CCL4 protein levels from samples including serum, plasma, and cell culture supernatants.

The Human CCL4 (MIP-1 beta) Uncoated ELISA Kit contains pre-matched antibody pairs,and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human CCL4 (MIP-1 beta). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.
TRUSTED_SUSTAINABILITY

Spezifikation

Zugriffsnummer P13236
Assay-Bereich 4-500 pg/mL
Assay-Empfindlichkeit: 4pg/mL
Inhalt und Lagerung Capture-Antikörper: Vortitrierter, aufgereinigter Antikörper
Nachweisantikörper: Vortitrierter, biotinkonjugierter Antikörper
Standard: Rekombinantes Protein zur Erzeugung von Standardkennlinien und Probenkalibrerung
10 X Beschichtungspuffer: Puffer für die Beschichtung des Capture-Antikörpers
5 X ELISA/ELISPOT-Verdünnungsmedium: Puffer zum Blockieren und Verdünnen des Nachweisantikörpers und -enzyms
Enzym: Vortitriertes Streptavidin-HRP
Substrat: 1 X TMB-Lösung
Analysenzertifikat: Chargenspezifische Anweisungen für die Verdünnung von Antikörpern und Standards
96-Well-Platte: Corning Costar 9018 (im Lieferumfang von Produkt-Kat.- Nrn. enthalten, die auf -22, -76, -86 enden)
Konjugat HRP
Immunassay-Kit-Format Uncoated ELISA Kit
Produkttyp ELISA
Probentyp Plasma, Serum, Supernatant
Zielspezies Human
Zur Verwendung mit (Anwendung) ELISA
Zur Verwendung mit (Geräte) Absorbance Microplate Reader
Gen-Alias ACT2; ACT-2; AT744.1; CC chemokine ligand 4; C-C motif chemokine 4; C-C motif chemokine ligand 4; Ccl4; chemokine (C-C motif) ligand 4; G-26; G-26 T-lymphocyte-secreted protein; HC21; immune activation gene 2; immune activation gene-2; immune activation protein 2; LAG1; LAG-1; Lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; macrophage inflammatory protein-1 beta; MGC104418; MGC126025; MGC126026; MIP-1 beta; MIP1b; MIP-1B; Mip1-b; MIP1B1; MIP1beta; MIP-1BETA; MIP-1-beta; MIP1-beta; MIP-1-beta(1-69); MIP-1-beta(3-69); PAT 744; Protein H400; putative MIP-1beta protein; RP23-320E6.8; SCY4A; SCYA2; Scya4; secreted protein G-26; SIS-gamma; small inducible cytokine A4; small inducible cytokine A4 (homologous to mouse Mip-1b); small-inducible cytokine A4; T-cell activation protein 2ACT2; ACT-2; AT744.1; CC chemokine ligand 4; C-C motif chemokine 4; C-C motif chemokine ligand 4; Ccl4; chemokine (C-C motif) ligand 4; G-26; G-26 T-lymphocyte-secreted protein; HC21; immune activation gene 2; immune activation gene-2; immune activation protein 2; LAG1; LAG-1; Lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; macrophage inflammatory protein-1 beta; MGC104418; MGC126025; MGC126026; MIP-1 beta; MIP1b; MIP-1B; Mip1-b; MIP1B1; MIP1beta; MIP-1BETA; MIP-1-beta; MIP1-beta; MIP-1-beta(1-69); MIP-1-beta(3-69); PAT 744; Protein H400; putative MIP-1beta protein; RP23-320E6.8; SCY4A; SCYA2; Scya4; secreted protein G-26; SIS-gamma; small inducible cytokine A4; small inducible cytokine A4 (homologous to mouse Mip-1b); small-inducible cytokine A4; T-cell activation protein 2; ACT2; AT744.1; G-26; HC21; LAG-1; LAG1; MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4
Gen-ID (Entrez) 6351
Gensymbol CCL4
Kitinhalt Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Marker oder Farbstoff Biotin
Menge 10 x 96 Tests
Kennzeichnung RUO
Lagerungsbedingungen 2 bis 8 °C außer Standard, der bei ≤−70 °C gelagert werden sollte
Synonym C-C Motif-Chemokin 4, Macrophage Inflammatory Protein-1 beta
Ziel CCL4 (MIP-1 beta)
Prüfzeit 1 hr 45 min
Gesamte Assay-Zeit 24 hr 30 min
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