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PeproTech CCL5 (RANTES) Polyclonal Antibody, PeproTech®, Invitrogen™

Artikelnummer. 17853583
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50 μg
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1 Milligramm
100 Mikrogramm
50 Mikrogramm
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Artikelnummer. 17853583 Lieferant PeproTech Lieferanten-Nr. 500P7850UG

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Beschreibung

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat RANTES (CCL5). Anti-Rat RANTES (CCL5)-specific antibody was purified by affinity chromatography employing an immobilized Rat RANTES (CCL5) matrix. Sandwich ELISA: To detect Rat RANTES (CCL5) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat RANTES (CCL5) (500-P78Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat RANTES (CCL5). Western Blot: To detect Rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat RANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.
TRUSTED_SUSTAINABILITY

Spezifikation

Antigen CCL5 (RANTES)
Anwendungen ELISA, Western Blot
Klassifikation Polyclonal
Konzentration 0.1-1.0 mg/mL
Konjugat Unconjugated
Zusammensetzung PBS with no preservative
Gen CCL5
Gen-Zugriffsnummer P50231
Gen-Alias beta-chemokine RANTES; CC chemokine; C-C motif chemokine 5; C-C motif chemokine ligand 5; CCL5; chemokine (C-C motif) ligand 5; D17S136E; EoCP; eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; RANTES; RANTES protein; RANTES(3-68); RANTES(4-68); regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; Scya5; SIS δ; SISd; SIS-delta; SISδ; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228; Unknown (protein for MGC:127014)
Gensymbole CCL5
Wirtsspezies Rabbit
Immunogen E.coli-derived Recombinant Rat RANTES (CCL5).
Reinigungsverfahren Antigen affinity chromatography
Menge 50 μg
Regulatorischer Status RUO
Primär oder sekundär Primary
Gen-ID (Entrez) 81780
Zielspezies Rat
Inhalt und Lagerung -20°C
Produkttyp Antibody
Form Lyophilized
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