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CD103 (Integrin alpha E) Armenian Hamster anti-Mouse, Super Bright 600, Clone: 2E7, eBioscience

Armenian Hamster Monoclonal Antibody

Marke:  Affymetrix eBioscience 63-1031-80

Weitere Details : Netto-Gewicht : 0.01000kg

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Artikelnummer. 16331444

  • 137.00 € / 2 Stück

Voraussichtliches Versanddatum: 08-11-2021

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Beschreibung

Beschreibung

Description: The 2E7 monoclonal antibody reacts with mouse CD103, the alpha-E integrin. CD103 non-covalently associates with integrin beta7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Applications Reported: This 2E7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2E7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions.

Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs.
Spezifikation

Spezifikation

CD103 (Integrin alpha E)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Q60677
Itgae
IgG
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16407
Flow Cytometry
2E7
Super Bright 600
Itgae
Liquid
Armenian Hamster
Affinity Chromatography
RUO
Antibody
Monoclonal
Mouse
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation
Zertifikate