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CD115 (c-fms) Rat anti-Mouse, Super Bright 600, Clone: AFS98, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 63-1152-82

Weitere Details : Netto-Gewicht : 0.01000kg

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Artikelnummer. 15913534

  • 316.00 € / 100 Mikrogramm
Voraussichtliches Versanddatum: 08-12-2021

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Beschreibung

Beschreibung

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage. Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No.

SB-4401) to minimize any non- specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid maligcy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction.
Spezifikation

Spezifikation

CD115 (c-fms)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P09581
AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
AFS98
Super Bright 600
Csf1r
Liquid
Csf1r
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12978
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation
Zertifikate
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