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CD247 (CD3 zeta) Mouse anti-Human, APC, Clone: 6B10.2, eBioscience

Mouse Monoclonal Antibody

Marke:  Affymetrix eBioscience 17-2479-42

Weitere Details : Netto-Gewicht : 0.01000kg

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Artikelnummer. 16340694

  • 317.00 € / 100 Tests

Voraussichtliches Versanddatum: 09-11-2021

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Beschreibung

Beschreibung

Description: This 6B10.2 monoclonal antibody reacts with human CD247, which is also known as the invariant zeta chain. The CD3 complex is composed of three monomeric chains (gamma, delta, and epsilon) and the invariant zeta chain, which exists as a homodimer or as a heterodimer with the epsilon subunit. Together with the T cell antigen receptor (TCR), the CD3 gamma, delta, epsilon, and zeta chains comprise the TCR/CD3 complex. CD3 zeta is expressed on immature and mature thymocytes, as well as T lymphocytes and NK cells. Signal transduction during T cell activation is mediated by tyrosine phosphorylation of cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) present on the zeta chain. The 6B10.2 monoclonal antibody recognizes a transmembrane epitope of the CD3 zeta chain. Therefore, flow cytometric analysis with this antibody requires intracellular staining. In addition, this antibody has been reported to recognize the zeta chain as a homodimer but not as a monomer. Applications Reported: This 6B10.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6B10.2 antibody has been pre-diluted and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol.

Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at thermofisher.com. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Spezifikation

Spezifikation

CD247 (CD3 zeta)
Monoclonal
5 μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P20963
CD247; CD3-ZETA; CD3H; CD3Q; CD3Z; IMD25; T3Z; TCRZ
Mouse
Affinity Chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
6B10.2
APC
CD247
Liquid
CD247
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
919
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation
Zertifikate