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CD28 Monoclonal Antibody (CD28.2), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

53.50 € - 104.00 €

Spezifikation

Antigen CD28
Klon CD28.2
Konzentration 0.5 mg/mL
Anwendungen Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Klassifikation Monoclonal
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Produkte 2
Produktcode Marke Menge Preis Menge & Verfügbarkeit  
Produktcode Marke Menge Preis Menge & Verfügbarkeit  
15236887
Unterlagen ansehen Aktionsdetails
Affymetrix eBioscience
14-0289-80
25 μg
53.50 €
25 Mikrogramm
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Verfügbar ab: 08-05-2024
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15246887
Unterlagen ansehen Aktionsdetails
Affymetrix eBioscience
14-0289-82
100 μg
104.00 €
100 Mikrogramm
Alternative Produkte anzeigen
Sparen Sie bis zu 
»
Lager vorrätig
Sie sich ein, um den Lagerbestand zu sehen
Bitte Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute!
Beschreibung

Beschreibung

Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. The recommended titer for immunoprecipitation is ∽2-5 μg per million cells. CD28.2 has also been reported in costimulation of T cells in in vitro functional assays. (Please use Functional Grade purified CD28.2, cat. 16-0289, in functional assays.). Applications Tested: The CD28.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
Spezifikation

Spezifikation

CD28
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
antigen CD28; CD28; CD28 antigen; CD28 antigen (Tp44); CD28 isoform; CD28 isoform 2; Cd28 molecule; CD28 precursor protein; CD28 protein; CD28 protein precursor; CD28RNA; cell surface protein; CHT28; contains partial extracellular domain; costimulatory molecule B7 receptor CD28; EGK_04709; MGC138290; sCD28; soluble CD28; T44; T-cell costimulatory molecule CD28; T-cell costimulatory molecule Tp44; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28 homolog; TP44
Cd28
Primary
4° C
Cd28
CD28.2
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Unconjugated
Mouse
Human
P10747
940
IgG1 κ
Affinity chromatography
Antibody
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Produktzertifizierungen

For Research Use Only.