CD43 Monoclonal Antibody (4-29-5-10-21), APC, eBioscience™, Invitrogen™

Beschreibung

Description: The monoclonal antibody 4-29-5-10-21 reacts with human and non-human primate CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which, like many mucins, can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes and a small subset of B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. Applications Reported: This 4-29-5-10-21 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 4-29-5-10-21 antibody has been pre-titrated and tested by flow cytometric anaylsis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.