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Invitrogen™ Corticosterone Competitive ELISA Kit
Competitive ELISA
Marke: Invitrogen™ EIACORT
Weitere Details : Netto-Gewicht : 0.33820kg Transport : UN number : 1789 Chem class : 8 Pack group : II
Beinhaltet: Vorbeschichtete Platte(n), Standard, Nachweisantikörper, HRP-Konjugat, Assay-Puffer, Waschpuffer, Chromogen, Stopplösung, Plattenabdeckungen und chargenspezifisches technisches Datenblatt.
Beschreibung
The Corticosterone ELISA quantitates coritcosterone in serum, plasma, urine, dried fecal extracts or cell culture medium. The assay will exclusively recognize both natural and recombinant coritcosterone. Principle of the method The corticosterone solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Corticosteron ist ein adrenokortikales Steroid, das mäßige aber dennoch bedeutende Aktivität als Mineralocorticoid und Glucocorticoid aufweist. [National Center for Biotechnology Information. PubChem Compound Database; CID=5753].Spezifikation
| 78.125 bis 10,000 pg/ml | |
| Corticosterone Competitive ELISA Kit | |
| HRP | |
| ELISA-Kit | |
| Fäkalien Extrakt, Plasma, Serum, Überstand, Urin | |
| Human | |
| Kolorimetrisches Mikrotiterplatten-Lesegerät | |
| 5.2 % | |
| HRP | |
| RUO | |
| Stuhlextrakt, 50 μl; Plasma, 1 μl; Serum, 1 μl; Überstand, 50 μl; Urin, 2,5 μl | |
| Chemikalien | |
| 1 Std 30 Min |
| 18,6 pg/mL | |
| Multiplex-Assay auf der Luminex Plattform | |
| Fluoreszent | |
| Steroidhormon | |
| 96 Assays | |
| ELISA, Protein-Assays, Aktivitäts-Assays, Proteinbiologie, Reproduktive Biologie, Entzündungen, Oxidativer Stress, Metabolismus, Zellsignalweiterleitung, Epigenetik, Hämatologie, DNA-Schaden, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Steroid-Hormon-Nachweis | |
| 7.9 % | |
| Vorbeschichtete 96-Well-Platte, Assay-Verdünnungskonzentrat, HRP-konjugierter Nachweisantikörper., Waschpuffer, Stopplösung, Dichtungsfolien für Mikrotiterplatten, Stopplösung, Dichtungsfolien für Mikrotiterplatten | |
| 96 Tests | |
| Entzündungen | |
| −20 °C | |
| 1 Std 20 Min |