T Lymphocyte Cocktail Mouse anti-Dog, APC, FITC, PE, Clone: LSM 8.358, LSM 1.140, LSM 12.125, BD

Cocktail Components Clone Isotype

APC anti-Dog Pan T Cell LSM 8.358 mIgM

FITC anti-Dog CD8 LSM 1.140 mIgG1

The Dog T Lymphocyte Cocktail is a three-color reagent cocktail designed to identify dog T lymphocytes by direct immunofluorescence staining with flow cytometric analysis. The LSM 1.140 antibody reacts with a CD8-like 32-35 kDa heterodimer. The CD8α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopuplation of mature T-lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). The LSM 12.125 antibody reacts with the canine form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal canine donor peripheral blood lymphocytes. The distribution on lymphocytes for canine is similar to that found for both human and monkey, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers. CD4 in the canine is also present on monocytes and granulocytes, but in higher numbers than what is seen for human. The LSM 8.358 antibody reacts with a CD3-like T-cell receptor-associated cell-surface antigen found on thymocytes and peripheral T lymphocytes. These monoclonals were generated by immunizing mice with freshly isolated canine thymocytes.