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EOMES Rat anti-Mouse, PE, Clone: Dan11mag, eBioscience™

Rat Monoclonal Antibody

Marke:  Affymetrix eBioscience 12-4875-82

Weitere Details : Netto-Gewicht : 0.25000kg

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Artikelnummer. 15258789

  • 330.00 € / 100 Mikrogramm

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Beschreibung

Beschreibung

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R. Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This Dan11mag antibody has been tested by intracellular flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
Spezifikation

Spezifikation

Eomes
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O54839
Eomesodermin homolog; EOMES; TBR2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
Dan11mag
PE
Eomes
Liquid
Eomes
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
13813
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.