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Invitrogen™ Estrone-3-Glucuronide (E1G) Competitive ELISA Kit
Competitive ELISA
Marke: Invitrogen™ EIA17E3
Weitere Details : Netto-Gewicht : 0.33820kg
Beinhaltet: Vorbeschichtete Platte(n), Standard, Nachweisantikörper, HRP-Konjugat, Assay-Puffer, Waschpuffer, Chromogen, Stopplösung, Plattenabdeckungen und chargenspezifisches technisches Datenblatt.
Beschreibung
The Estrone-3 Glucuronide (E1G) ELISA quantitates estrone-3 glucuronide in extracted serum,plasma,urine,dried fecal extracts or cell culture medium.
Principle of the method
The Estrone-3-Sulfate (E1S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Estrone-3-Sulfate (E1S) in a variety of matrices,including serum,plasma,urine,and fecal samples. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Spezifikation
| 15.6 bis 1000 pg/ml | |
| Estron-3 -Glucuronid (E1 G) Competitive ELISA Kit | |
| HRP | |
| ELISA-Kit | |
| Fäkalien Extrakt, Serum, Überstand, Urin | |
| Human | |
| Kolorimetrisches Mikrotiterplatten-Lesegerät | |
| 3.8 % | |
| HRP | |
| RUO | |
| Stuhlextrakt, 50 μl; Serum, 50 μl; Überstand, 50 μl; Urin, 6,25 μl | |
| Chemikalien | |
| 2 Std 30 Min |
| 7,38 pg/mL | |
| Multiplex-Assay auf der Luminex Plattform | |
| Fluoreszent | |
| Steroidhormon | |
| 96 Assays | |
| ELISA, Protein-Assays, Aktivitäts-Assays, Proteinbiologie, Reproduktive Biologie, Entzündungen, Oxidativer Stress, Metabolismus, Zellsignalweiterleitung, Epigenetik, Hämatologie, DNA-Schaden, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Steroid-Hormon-Nachweis | |
| 5.6 % | |
| Vorbeschichtete 96-Well-Platte, Assay-Verdünnungskonzentrat, HRP-konjugierter Nachweisantikörper., Waschpuffer, Stopplösung, Dichtungsfolien für Mikrotiterplatten, Stopplösung, Dichtungsfolien für Mikrotiterplatten | |
| 96 Tests | |
| Reproduktive Biologie | |
| −20 °C | |
| 1 Std 20 Min |