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  7. Gibco™ DMEM/F-12 

Gibco™ DMEM/F-12
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Product Code. 11554546
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Quantity:
500 mL
Unit Size:
500mL
Product Code. Quantity unitSize
11554546 500 mL 500mL
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For Research Use Only. All usage must comply with product instructions.

Product Code. 11554546

Brand: Gibco™ 11320033

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24.13 EUR valid until 2026-02-28
BEST PRICE on Promo! Use promo code "28001" to get your promotional price.
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using DMEM/F-12

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

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Specifications

Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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