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GL7 Monoclonal Antibody (GL-7 (GL7)), eFluor™ 660, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Marke: Affymetrix eBioscience 50-5902-82
Weitere Details : Netto-Gewicht : 0.50000kg
Beschreibung
Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This GL-7 (GL7) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
GL7 Monoclonal antibody specifically detects GL7 in Human, Mouse samples. It is validated for Flow Cytometry.Spezifikation
| GL7 | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Rat | |
| 100 μg | |
| Primary | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| GL-7 (GL7) | |
| eFluor 660 | |
| GL7 antigen; Ly77; Ly-77 | |
| Affinity chromatography | |
| RUO | |
| Human, Mouse | |
| Antibody | |
| IgM |
For Research Use Only.