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Invitrogen™ GSK-3A Human Protein

Easily screen for kinase binding. Thermo Fisher Invitrogen™ GSK-3A Human Protein recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.

1826.00 € - 7890.00 €

Spezifikation

Zugriffsnummer NP_063937.2
Zur Verwendung mit (Anwendung) Enzyme and Protein Activity Assay, Kinase Assay, Protein Assays and Analysis, Protein Biology
Zusammensetzung 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol.
Molekulargewicht 78.4kDa
Lagerungsbedingungen Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
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Produkte 2
Produktcode Marke Menge Preis Menge & Verfügbarkeit  
Produktcode Marke Menge Preis Menge & Verfügbarkeit  
15428723
Unterlagen ansehen Aktionsdetails
Invitrogen™
PR9188A
100μg
1826.00 €
100 Mikrogramm
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Verfügbar ab: 16-05-2024
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15428813
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Invitrogen™
PV6127
1mg
7890.00 €
1 Milligramm
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Verfügbar ab: 23-04-2024
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Beschreibung

Beschreibung

The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.

  • Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
  • Expressed in insect cells
  • GST tagged for use with Eu-labeled GST antibody
  • No special measures were taken to activate this kinase

GSK3A for the LanthaScreen Kinase Binding Assay
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)

This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 236 and Eu-labeled GST reagents.

Spezifikation

Spezifikation

NP_063937.2
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol.
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
Full Length
GSK Kinase Family
Recombinant
Baculovirus
Enzyme and Protein Activity Assay, Kinase Assay, Protein Assays and Analysis, Protein Biology
78.4kDa
RUO - research use only
GSK-3A
Human
GST Tag
Frozen
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Produktzertifizierungen