Invitrogen™ ADAM11/MDC Human ELISA Kit

Beschreibung

The Human ADAM11/MDC ELISA quantitates Hu ADAM11 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ADAM11. Principle of the method The Human ADAM11 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Dieses Gen kodiert ein Mitglied der ADAM-Proteinfamilie (ein Disintegrin und Metalloprotease). Mitglieder dieser Familie sind membranverankerte Proteine, die strukturell mit dem Zerfall von Schlangengiften zusammenhängen und an einer Vielzahl von biologischen Prozessen beteiligt sind, die Zelle-zu-Zelle- und Zell-Matrix-Interaktionen beinhalten, einschließlich Befruchtung, Muskelaufbau und Neurogenese. Dieses Gen ist ein Tumorsuppressorkandidat für humanen Brustkrebs, basierend auf seiner Position innerhalb einer minimalen Region von Chromosom 17q21, die zuvor durch Tumordeletionskartierung definiert wurde.