Invitrogen™ Angiopoietin 1 Human ELISA Kit

Beschreibung

The Human Angiopoietin 1 (Hu APO1) ELISA quantitates Hu APO1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APO1. Principle of the method The Human APO1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Angiopoietin-1 (Ang-1) ist ein sezernierter Ligand für Tie-2. Vorläufige biochemische Analysen von Ang-1 zeigen eine potenzielle Fibrinogen-ähnliche Domäne am Carboxy-Terminus und Coiled-Coil-Domänen im Amino-Terminus. Ang-1 ist ein angiogener Faktor, der an der endothelialen Entwicklung beteiligt ist. Ein verwandtes Protein, Angiopoietin-2 (Ang-2), wurde als natürlich vorkommender Antagonist der Aktivierung von Tie-2 durch Ang-1 identifiziert. Im Erwachsenengewebe scheint die Ang-2-Expression auf die Stellen der vaskulären Remodellierung beschränkt zu sein.