Invitrogen™ Cathepsin S/CTSS Human ELISA Kit

Beschreibung

The Human Cathepsin S (CTSS) ELISA quantitates Hu Cathepsin S in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Cathepsin S. Principle of the method The Human Cathepsin S solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Das von diesem Gen codierte Protein, ein Mitglied der Peptidase C1-Familie, ist eine lysosomale Cystein-Proteinase, die möglicherweise am Abbau antigener Proteine zu Peptiden für die Präsentation auf MHC-Klasse-II-Molekülen beteiligt ist. Das codierte Protein kann über einen breiten pH-Bereich als Elastase in alveolären Makrophagen fungieren. Für dieses Gen existieren Transkriptvarianten, die alternative Polyadenylierungssignale verwenden.