R&D Systems™ Human Cytochrome c Quantikine ELISA Kit, R&D Systems™
a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma.
Marke: R&D Systems™ DCTC0
Weitere Details : Netto-Gewicht : 0.01000kg
BeschreibungThis assay employs the quantitative sandwich enzyme immunoassay technique and is designed to replace a Western Blot. A monoclonal antibody specific for human Cytochrome c has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cytochrome c present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human Cytochrome c is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cytochrome c bound in the initial step. The color development is stopped and the intensity of the color is measured.
|0.63 - 20ng/mL|
|<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules|
|Cell Lysates (20μL), Subcellular Fractions (100μL)|
|Store the unopened product at 2 - 8° C. Do not use past expiration date.|
|Solid Phase Sandwich ELISA|
|Cell Lysates (20uL), Subcellular Fractions (100uL)|
|Sufficient materials to run ELISAs on at least one 96-well plate|