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R&D Systems™ Human Cytochrome c Quantikine ELISA Kit, R&D Systems™

a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma.

Marke:  R&D Systems™ DCTC0

Weitere Details : Netto-Gewicht : 0.01000kg

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Artikelnummer. 13412625

  • 760.28 € / 96 Tests


Voraussichtlicher Liefertermin 30-04-2019
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Beschreibung

Beschreibung

This assay employs the quantitative sandwich enzyme immunoassay technique and is designed to replace a Western Blot. A monoclonal antibody specific for human Cytochrome c has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cytochrome c present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for human Cytochrome c is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cytochrome c bound in the initial step. The color development is stopped and the intensity of the color is measured.
Spezifikation

Spezifikation

0.63 - 20ng/mL
<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules
54205
1 Plate
0.31ng/mL
Cell Lysates (20μL), Subcellular Fractions (100μL)
Store the unopened product at 2 - 8° C. Do not use past expiration date.
HRP
ELISA
Solid Phase Sandwich ELISA
1 Kit
Cell Lysates (20uL), Subcellular Fractions (100uL)
Human
Sufficient materials to run ELISAs on at least one 96-well plate
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