Invitrogen™ D-Dimer Human ELISA Kit

Beschreibung

The Human D-Dimer ELISA quantitates Hu D-Dimer in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu D-Dimer. Principle of the method The Human D-Dimer solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Fibrinogen ist das Hauptprotein des Blutgerinnungssystems. Es besteht aus zwei identischen Untereinheiten, die drei Polypeptidketten enthalten: Alpha, Beta und Gamma. Der Prozess der Blutgerinnung führt zur Aktivierung von Fibrinogen zu Fibrin durch Thrombin- und Fibrinpolymerisation. Das Fibringerinnsel wird dann von Plasmin verdaut, und Fibrin-Abbauprodukte mit unterschiedlichen Molekulargewichten werden in den Blutkreislauf abgegeben.