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Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience™, Invitrogen™
Polyclonal Antibody
93.50 € - 172.00 €
Spezifikation
Antigen | Fc Receptor Binding Inhibitor |
---|---|
Konzentration | 1 mg/mL |
Anwendungen | Flow Cytometry |
Klassifikation | Polyclonal |
Konjugat | Unconjugated |
Benachrichtigungen:
Contains Azide
Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
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Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
15218157
|
Affymetrix eBioscience
14-9161-71 |
25 Tests |
93.50 €
25 Tests |
Lager vorrätigLoggen Sie sich ein, um den Lagerbestand zu sehen | Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
15228157
|
Affymetrix eBioscience
14-9161-73 |
100 Tests |
172.00 €
100 Tests |
Lager vorrätigLoggen Sie sich ein, um den Lagerbestand zu sehen | Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
Beschreibung
Description: The human FcgammaR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcgammaR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcgammaR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcgammaRs. Applications Reported: Purified Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis. To inhibit the non-specific binding of mouse monoclonal antibodies add the human Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody. Applications Tested: Purified human Fc gammaR-binding inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 μL per test.
Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.Spezifikation
Fc Receptor Binding Inhibitor | |
Flow Cytometry | |
Unconjugated | |
Human | |
Human | |
Fc blocking; FcR BI; FcR Inhibitor | |
Affinity chromatography | |
Antibody |
1 mg/mL | |
Polyclonal | |
Liquid | |
RUO | |
PBS with 0.09% sodium azide; pH 7.2 | |
Primary | |
4° C |
For Research Use Only