Invitrogen™ eBioscience™ Platin-ELISA-Kit für humanes Granzym B

Beschreibung

The Human Granzyme B ELISA quantitates Hu Granzyme B in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme B. Principle of the method The Human Granzyme B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

• Die prozentualen Anteile der Granzym A- und B-positiven CTLs können mittels Durchflusszytometrie und immunzytochemischer Methoden für viele Erkrankungen bestimmt werden
• Nicht alle Granzyme gelangen in die Zielzelle, ein Teil von ihnen geht auch in das periphere Blut und andere biologische Flüssigkeiten über
• Nachweisbare Mengen an Granzymen wurden bei gesunden Probanden im Körper vorgefunden
• Diese löslichen Granzyme können mit ELISAs gemessen werden