missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Reagenzien und Kits für Immunassays
  4. ELISA-Kits zur Life-Science-Forschung
  5. Invitrogen™ eBioscience™ Human IL-21 ELISA Ready-SET-Go!™ Kit, 2. Generation

Invitrogen™ eBioscience™ Human IL-21 ELISA Ready-SET-Go!™ Kit, 2. Generation

Artikelnummer. 15501217
Change view
Click to view available options
Menge:
10 x 96 Tests
20 x 96 Tests
2 x 96 Tests
Packungsgröße:
10 Stück
2 Stück
20 Stück
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Artikelnummer. Menge unitSize
15501217 20 x 96 Tests 20 Stück
15591207 2 x 96 Tests 2 Stück
15521217 10 x 96 Tests 10 Stück
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Dieser Artikel kann nicht zurückgegeben werden. Rückgaberichtlinie anzeigen
Enthält Natriumazid
Artikelnummer. 15501217 Lieferant Invitrogen™ Lieferanten-Nr. 88821876

Bitte Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute!

Dieser Artikel ist im Moment nicht verfügbar oder wird nicht mehr hergestellt.
Schauen Sie bitte auf unsere Produktseite nach möglichen Alternativen
 Alternative Produkte anzeigen

Dieser Artikel kann nicht zurückgegeben werden. Rückgaberichtlinie anzeigen
Enthält Natriumazid

Beschreibung

Speziell entwickelt für die genaue und präzise Messung von IL-21-Proteinkonzentrationen aus Proben, einschließlich Serum-, Plasma- und Zellkulturüberständen. Dieses Kit der zweiten Generation hat eine erhöhte Empfindlichkeit mit einem Bereich von 8 bis 1000 pg/ml.

The Human Interleukin 21 (IL-21) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-21. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.
TRUSTED_SUSTAINABILITY

Spezifikation

Zugriffsnummer Q9HBE4
Assay-Bereich 8-1000 pg/mL
Assay-Empfindlichkeit: 8pg/mL
Inhalt und Lagerung Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.
96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
Konjugat HRP
Immunassay-Kit-Format Uncoated ELISA Kit with Plates
Produkttyp ELISA
Probentyp Plasma, Serum, Supernatant
Zielspezies Human
Zur Verwendung mit (Anwendung) ELISA
Zur Verwendung mit (Geräte) Absorbance Microplate Reader
Gen-Alias CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11; BSF-1; IL-4
Gen-ID (Entrez) 59067
Gensymbol IL-21
Kitinhalt Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Marker oder Farbstoff Biotin
Protein-Aliase IL-21, Za11
Menge 20 x 96 Tests
Kennzeichnung RUO
Probenvolumen Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Lagerungsbedingungen 2 bis 8 °C außer Standard, der bei ≤−70 °C gelagert werden sollte
Synonym Interleukin-21
Ziel IL-21
Prüfzeit 1 hr 45 min
Gesamte Assay-Zeit 24 hr 30 min
Mehr anzeigen Weniger anzeigen

Nur für Forschungszwecke

Name des Produkts
Wählen Sie ein Thema

Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.