Invitrogen™ MIF Human ELISA Kit

Beschreibung

The Human Macrophage Inflammatory Factor (Hu MIF) ELISA quantitates Hu MIF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIF. Principle of the method The Human MIF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MIF (Macrophage Migration Inhibitory Factor, Hemmfaktor für die Makrophagen-Migration) ist ein Lymphokin, das an der zellvermittelten Immunität, Immunoregulation und Entzündung beteiligt ist. Es spielt eine Rolle bei der Regulation der Makrophagenfunktion bei der Abwehr des Wirts durch Unterdrückung der entzündungshemmenden Wirkungen von Glukokortikoiden. MIF kann auch eine Rolle bei Integrin-Signalwegen spielen, da es in der Nähe der peripheren Plasmamembran mit dem JAB1-Protein einen Komplex im Cytosol bildet.