Invitrogen™ MMP13 Human ELISA Kit

Beschreibung

The Human Matrix MetalloProteinase 13 (Hu MMP13) ELISA quantitates Hu MMP13 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MMP13. Principle of the method The Human MMP13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MMP13 (Kollagenase 3) ist an der Zersetzung extrazellulärer Matrixproteine, einschließlich fibrilläres Kollagen, Fibronektin, TNC und ACAN, beteiligt. MMP13 spaltet Tripelhelix-Kollagene, einschließlich Kollagen Typ I, II und III, hat jedoch die höchste Aktivität mit löslichem Kollagen des Typs II. Es kann Kollagen des Typs IV, XIV und X degradieren. MMP 13 ist bei Wundheilung, Gewebeumbildung, Knorpelabbau, Knochenentwicklung, Knochenmineralisierung und Ossifikation aktiv. Es wird für die normale embryonale Knochenentwicklung und Ossifikation benötigt. Mutationen, die das Gen beeinflussen, können zu Spondyloepimetaphysäre Dysplasie (Missouri-Typ), Metaphyseal Anadysplasia 1 und Metaphysäre Dysplasie (Sphar-Typ) führen.