Invitrogen™ Serpin A4 Human ELISA Kit

Beschreibung

The Human Serpin A4 ELISA quantitates Hu Serpin A4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Serpin A4. Principle of the method The Human Serpin A4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Hemmt die Aktivitäten der humanen amidolytischen Aktivität und Kininogenase von Gewebekallikrein. Die Hemmung wird durch die Bildung eines äquimolaren, hitze- und SDS-stabilen Komplexes zwischen dem Inhibitor und dem Enzym und durch die Spaltung an der reaktiven Stelle durch Gewebekalliklurein durch die Bildung eines kleinen C-terminalen Fragments des Inhibitors erreicht.