Invitrogen™ DIABLO/SMAC Human ELISA Kit

Beschreibung

The Human DIABLO (Hu SMAC) ELISA quantitates Hu SMAC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SMAC. Principle of the method The Human SMAC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Apoptose-Protein-Inhibitoren (IAP) blockieren den Zelltod durch Kaspaseinhibition. Der X-chromosale Apoptose-Hemmer (XIAP) ist ein Mitglied der IAP-Familie. Smac/Diablo wird in einer Vielzahl von Geweben exprimiert und hat sich als negativer XIAP-Regler erwiesen. Smac/Diablo ist ein mitochondriales Protein, das bei einer Zell-Apoptose in das Zytosol eindringt, und mindert die Kaspashemmung durch die IAP.