Invitrogen™ TACE/ADAM17 Human ELISA Kit

Beschreibung

The Human TACE/ADAM17 ELISA quantitates Hu TACE in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TACE. Principle of the method The Human TACE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Spaltet den membrangebundenen Vorläufer von TNF-alpha in seine reife lösliche Form. Verantwortlich für die proteolytische Freisetzung von löslichem JAM3 aus der Endothelzelloberfläche. Verantwortlich für die proteolytische Freisetzung mehrerer weiterer Zelloberflächenproteine, einschließlich p75 TNF-Rezeptor, Interleukin1-Rezeptor Typ II, p55 TNF-Rezeptor, transformierender Wachstumsfaktor-alpha, L-Selectin, Wachstumshormonrezeptor, MUC1 und das Amyloid-Vorläuferprotein. Dient als Aktivator des Notch-Pfades durch Vermittlung der Notch-Spaltung, wodurch das membranassoziierte Zwischenfragment namens Notch abgeschieden wird.