Invitrogen™ TGFBI (BIGH3) Human ELISA Kit

Beschreibung

The Human Transforming Growth Factor beta1(TGFβ1) (BIGH3) ELISA quantitates Hu TGFβ1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFβ1. Principle of the method The Human TGFβ1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Dieses Gen kodiert ein RGD-haltiges Protein, das an Kollagene der Typen I, II und IV bindet. Das RGD-Motiv findet sich in vielen extrazellulären Matrixproteinen, die die Zelladhäsion modulieren, und dient als Ligandenerkennungssequenz für mehrere Integrine. Dieses Protein spielt eine Rolle bei Zellkollagen-Interaktionen und kann an der endochondrialen Knochenbildung im Knorpel beteiligt sein. Das Protein wird durch Umwandlung des beta-Wachstumsfaktors induziert und behindert die Zelladhäsion. Mutationen in diesem Gen sind mit mehreren Arten von Hornhautdystrophie assoziiert.