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Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 405, Invitrogen™

Donkey Polyclonal Secondary Antibody

Marke:  Thermo Scientific A48268

354.35 EUR gültig bis 2024-03-29
Benutzen Sie den Promotions-Code "21653" um Ihren Promotions-Preis zu erhalten.



Weitere Details : Netto-Gewicht : 0.01000kg

Artikelnummer. 17206543

  • 373.00 € / 1 Milligramm
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Beschreibung

Beschreibung

To minimize cross-reactivity, the donkey anti-rat IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, goat, rabbit, mouse, chicken, guinea pig, hamster, horse, sheep, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. Specificity: This antibody binds to heavy chains on mouse IgG and light chains on all rat immunoglobulins. This antibody does not bind non-immunoglobulin rat serum proteins or IgG from bovine, goat, rabbit, mouse, chicken, guinea pig, hamster, horse, sheep, or human.

To minimize cross-reactivity, the donkey anti-rat IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, goat, rabbit, mouse, chicken, guinea pig, hamster, horse, sheep, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. Specificity: This antibody binds to heavy chains on rat IgG and light chains on all rat immunoglobulins. This antibody does not bind non-immunoglobulin rat serum proteins or IgG from bovine, goat, rabbit, mouse, chicken, guinea pig, hamster, horse, sheep, or human.
Spezifikation

Spezifikation

Rat IgG (H+L) Highly Cross-Adsorbed
Polyclonal
Alexa Fluor Plus 405
Donkey
Affinity chromatography
RUO
Rat
Liquid
Immunocytochemistry
2 mg/mL
proprietary buffer with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone; pH 6.5
Gamma Immunoglobins Heavy and Light chains
1 mg
Secondary
4° C, store in dark
IgG
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Zertifikate
Sonderangebote

Sonderangebote