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IgM Monoclonal Antibody (II/41), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

69.00 € - 180.00 €

Spezifikation

Antigen IgM
Klon II/41
Konzentration 0.5 mg/mL
Anwendungen ELISA, Flow Cytometry, Immunohistochemistry (Frozen)
Klassifikation Monoclonal
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Produkte 3
Produktcode Marke Menge Preis Menge & Verfügbarkeit  
Produktcode Marke Menge Preis Menge & Verfügbarkeit  
15217477
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Affymetrix eBioscience
14-5790-82
100 μg
120.00 €
100 Mikrogramm
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Verfügbar ab: 08-05-2024
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15227477
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Affymetrix eBioscience
14-5790-85
500 μg
180.00 €
500 Mikrogramm
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15207477
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Affymetrix eBioscience
14-5790-81
50 μg Dieser Artikel ist im Moment nicht verfügbar oder wird nicht mehr hergestellt.
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Beschreibung

Beschreibung

Description: The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM. Applications Reported: The II/41 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA. Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
Spezifikation

Spezifikation

IgM
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH
Ighm
Primary
4° C
Ighm
II/41
ELISA, Flow Cytometry, Immunohistochemistry (Frozen)
Unconjugated
Rat
Mouse
P01872
16019
IgG2a κ
Affinity chromatography
Antibody
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Produktzertifizierungen

For Research Use Only.