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Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit
Beskrivelse
Indeholder
Resuspension Buffer (R3)
RNase A (20 mg/mL)
Lysis Buffer (L7)
Precipitation Buffer (N4)
Wash Buffer (W9)
Wash Buffer (W10)
TE Buffer (TE)
Spin Columns
Wash and Recovery Tubes
The PureLink™ Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in 30-45 minutes, using silica membrane columns.
Features of the PureLink™ Quick Plasmid Miniprep Kit:
- Fast—ready-to-use plasmid DNA typically in 30-45 minutes
- Flexible—formats work with vacuum and centrifugation platforms
Reliable and cost-effective isolation of plasmid DNA
The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30-45 minutes, using the PureLink™ spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.
Compatible with flexible formats
The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A260/A280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.
Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.
Ordreinfo
Shipping Condition: Room Temperature
Tekniske data
Tekniske data
| Concentration | 20 mg/mL |
| Column Type | Spin Column |
| Content And Storage | • Resuspension buffer • RNase A (20 mg/mL) • Lysis buffer • Precipitation buffer • Wash buffers • TE buffer • Spin columns in wash tubes • Elution tubes |
| Format | Spin Column |
| Isolation Technology | Silica Spin Column |
| Scale | Mini |
| Sample Type | Bacterial Culture |
| Final Product Type | Plasmid DNA |
| For Use With (Application) | Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
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Sikkerhed og håndtering
- PureLink Quick Plasmid Miniprep Kit
- Warning
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- MIXTURE LIST-Contains : Acetic acid
For Research Use Only. Not for use in diagnostic procedures.
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