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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli, no manual
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Menge:
40 Reaktionen
Packungsgröße:
1 Stück
Beschreibung
TA Cloning Kits use a pCR vector and yield ≥80% recombinants with ≥90% of the recombinants containing inserts.
- 3′-T overhangs for direct ligation of Taq-amplified PCR products
- Choice of T7 (pCR2.1) or T7 and Sp6 (pCRII) promoters for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- Choice of kanamycin or ampicillin selection
- Easy blue/white colony screening
- M13 forward and reverse primer sites for sequencing
- Manual not included
- Cloning Method: Restriction enzyme/MCS
Cloning, PCR Cloning
Spezifikation
Spezifikation
| Bakterien- oder Hefenstamm | INVαF' |
| Inhalt und Lagerung | Store One Shot E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Enthält | Linearized pCR2.1vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls, One Shot TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Without manual. |
| Anzahl Reaktionen | 40 |
| Produkttyp | One Shot TOP10 Chemically Competent E. coli |
| Menge | 40 Reaktionen |
| Vektor | TA Cloning Vectors |
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