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Ki-67 Mouse anti-Canine, Human, FITC, Clone: 20Raj1, eBioscience™

Mouse Monoclonal Antibody

Marke:  Affymetrix eBioscience 11-5699-82

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Artikelnummer. 15278239

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Beschreibung

Beschreibung

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells. This monoclonal antibody 20Raj1 recognizes canine Ki-67. Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 20Raj1 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.06 μg) per test.

A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
Spezifikation

Spezifikation

Ki-67
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P46013
antigen identified by monoclonal antibody Ki-67; antigen KI-67; KIA; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Human
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
20Raj1
FITC
MKI67
Liquid
MKI67
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
100686578, 4288
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.