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Ki-67 Rat anti-Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat, Alexa Fluor 488, Clone: SolA15, eBioscience

Rat Monoclonal Antibody

Marke:  Affymetrix eBioscience 53-5698-82

Weitere Details : Netto-Gewicht : 0.01000kg

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Artikelnummer. 16320994

  • 313.00 € / 1 Stück

Voraussichtliches Versanddatum: 15-11-2021

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Beschreibung

Beschreibung

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Refer to Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
Spezifikation

Spezifikation

Ki-67
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
E9PVX6, P46013
Mki67
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
100686578, 102135895, 17345, 291234, 4288
Flow Cytometry
SolA15
Alexa Fluor 488
MKI67
Liquid
Rat
Affinity Chromatography
RUO
Antibody
Monoclonal
Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation
Zertifikate