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MAP2K3 (MEK3) Recombinant Human Protein
MAP2K3 (MEK3) Recombinant Human Protein
Marke: Invitrogen PV3662
Weitere Details : Netto-Gewicht : 0.05250kg
Beschreibung
MAP2K3 (MEK3) is a dual-specificity protein kinase which acts in cellular signal transduction pathways, and is necessary for the expression of glucose transporter.Spezifikation
| NP_002747 | |
| Cell Viability, Pharma and Biopharma, Cell Proliferation and Function, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
| 64.2kDa | |
| 10μg | |
| For research use only. Not for use in diagnostic procedures | |
| MAP2K3 (MEK3) | |
| MAP2K3 was assayed in a two stage coupled reaction involving phosphorylation of inactive MAPK14 (PV3305) by MAP2K3 followed by phosphorylation of MBP substrate by activated MAPK14. The enzymes were diluted in enzyme dilution buffer before adding to the reaction. In stage one, MAP2K3 (8.33μg/mL final concentration) is incubated with MAPK14 (66.7μg/mL final concentration) in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, and 100μM ATP for 30 minutes at 30°C. In stage two, 5μL of the above reaction mix was transferred to 25μL of a reaction mixture containing 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100μM ATP, 20μg MBP substrate per reaction and trace [32P]-γ-ATP and incubated for 10 minutes at 30°C | |
| MAP2K3 (MEK3) Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| Serine/threonine kinase |
| 0.35mg/mL | |
| 5606 | |
| MAP2K3 (MEK3) Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| MAP2K3 (MEK3) Recombinant Human Protein | |
| STE7 | |
| 54nmoles of phosphate transferred to myelin basic protein (MBP) per minute per mg of total protein Activity determined at a final protein concentration of 16.67μg/mL | |
| GST Tag | |
| Kinase and inhibitors | |
| 90% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.