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MAPK9 (JNK2) Recombinant Human Protein, Inactive

MAPK9 (JNK2) Recombinant Human Protein, Inactive

Marke:  Invitrogen PV3621

Weitere Details : Netto-Gewicht : 0.05250kg

Artikelnummer. 11533477

  • 654.00 € / 100 Mikrogramm
Verfügbar ab: 30-04-2024
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Nur für Forschungszwecke. Die Anwendung muss den Produktanweisungen entsprechen.


Beschreibung

Beschreibung

MAPK9 (JNK2) is a serine⁄threonine kinase which plays an important role in the MAPK pathway.
Spezifikation

Spezifikation

NP_002743
Cell Viability, Pharma and Biopharma, Cell Proliferation and Function, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay
52.2kDa
100μg
For research use only. Not for use in diagnostic procedures
MAPK9 (JNK2)
Both the active and inactive enzymes were diluted in enzyme dilution buffer and the reaction was set up in two stages. In stage one, MAPK9, inactive (3.33μg/mL final concentration) was incubated with MAP2K7, mutant (0.83μg/mL final concentration) or with dilution buffer for basal MAPK9, inactive activity in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 2.5mM DTT, 0.5mM Na3VO4, 5mM β-glycerophosphate, 0.01% Triton™ X-100, 250μM ATP for 30 minutes at 30°C. In stage two, 5μL of the above reaction mix was transferred to 25μL of reaction mix containing 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 2.5mM DTT, 0.5mM Na3VO4, 5mM β- glycerophosphate, 0.01% Triton™ X-100, 200μM ATP, 167μg/mL ATF2 substrate and trace [32P]-γ-ATP and incubated for 10 minutes at 30°C
MAPK9 (JNK2) Recombinant Human Protein
Human
Baculovirus
Serine/threonine kinase
0.36mg/mL
5601
MAPK9 (JNK2) Recombinant Human Protein
–80°C
1 Each
MAPK9 (JNK2) Recombinant Human Protein
MAP
∽0% activity as compared to the active form of the kinase
His Tag
Kinase and inhibitors
90% by SDS-PAGE
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Zertifikate
Sonderangebote

Sonderangebote

For Research Use Only. Not for use in diagnostic procedures.