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Invitrogen™ Human MMP2, Recombinant Protein

Recombinant Protein

Marke:  Invitrogen™ RP77542

Weitere Details : CAS : 69-74-9 Netto-Gewicht : 0.01000kg

Artikelnummer. 11549091

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Beschreibung

Beschreibung

MMP2 Recombinant Protein, RP-77542, from Invitrogen.

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found. RP-77542 is expressed in Sf9-insect cells using the baculovirus expression vector system and purified from cell culture supernatant. The full-length recombinant proenzyme consists of 631 amino acids and has an apparent MW of 72 kDa. RP-77542 may be used as a control with corresponding anti-MMP-2 antibodies. Purity: 95% by SDS-PAGE The specific activity of human MMP2 (gelatinase A) after APMA activation is > 120 mU/mg. 1 U is the activity that hydrolyzes 1μmol peptide (7-methoxy-coumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L2,3-diaminopropionyl)- Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg) within 1 min. Gelatinase A is inhibited by tissue inhibitors of matrix metalloproteinases (TIMP), alpha2-macroglobulin and by chelators of divalent cations like EDTA or o-phenanthroline.
Spezifikation

Spezifikation

P08253
Control
4313
7.0
Insect cells
RUO
Human MMP2
Human
Hu
Recombinant
Recombinant
0.2mg/mL
50mM tris HCl with 0.05% Brij-35, 200mM NaCl, 5mM CaCl2 and no preservative; pH 7.0
Human MMP2
10μg
-80°C, Avoid Freeze/Thaw Cycles
Progelatinase A
MMP2
Unconjugated
Baculovirus
Liquid
purified
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