Invitrogen™ BLC/CXCL13 Mouse ELISA Kit

Beschreibung

The Mouse BLC/CXCL13 ELISA quantitates Ms BLC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BLC. Principle of the method The Mouse BLC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

B-Lymphozyten-Chemoattractant, unabhängig geklont und Angie genannt, ist ein CXC-Chemokin, das stark in den Follikeln der Milz, Lymphknoten und den Peyer-Plaques exprimiert wird. Es fördert bevorzugt die Migration von B-Lymphozyten (im Vergleich zu T-Zellen und Makrophagen), offenbar durch Anregung des Calciumzustroms und der Chemotaxis bei Zellen, die Burkit-Lymphom-Rezeptoren 1 exprimieren (BLR-1). Es kann daher beim Homing von B-Lymphozyten an Follikel unterstützen.