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Invitrogen™ Complement C5a Mouse ELISA Kit
Sandwich ELISA Kit
Marke: Invitrogen™ EMHC
Weitere Details : Netto-Gewicht : 0.00400kg
Beschreibung
The Mouse Complement C5a (HC) ELISA quantitates Ms Complement C5a in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Compelemnt C5a. Principle of the method The Mouse Complement C5a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
C5 ist Teil des Membranangriffskomplexes (MAC) des Komplementsystems. C5 wird durch die C5-Konvertasten über den klassischen und den alternativen Komplementweg in C5a und C5b aufgeteilt. Das C5b-Fragment initiiert den Aufbau des MAC-Terminus (C5b-C9). Die molekulare Masse von C5 beträgt 190 kDa.Spezifikation
| Gehäuse P06684 | |
| 1,5 pg/mL | |
| Festphasen-Sandwich-ELISA | |
| ELISA-Kit | |
| Plasma, Serum, Überstand | |
| 96 Assays | |
| ELISA, Protein-Assays, Proteinbiologie, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Proteinnachweis, Proteinanalyse | |
| 15139 | |
| < 12 % | |
| Vorbeschichtete 96-Well-Platte, Assay-Verdünnungskonzentrat, Biotinylierter Nachweisantikörper, Waschpuffer, Stopplösung, Dichtungsfolien für Mikrotiterplatten, Chromogen, Stopplösung, Dichtungsfolien für Mikrotiterplatten | |
| 96 Test-Kit | |
| Entwicklung | |
| 2 bis 8 °C | |
| Maus | |
| 4 Std 45 Min |
| 1.64 bis 100 pg/ml | |
| 1.5 bis 400 pg/ml | |
| Biotin | |
| Proteasen, Peptidasen und Inhibitoren | |
| Dieses ELISA-Kit zeigt keine Kreuzreaktivität mit den folgenden getesteten Zytokinen: Maus BLC, CD27, CD30 Ligand, Eotaxin, Eotaxin-2, Fas Ligand, G-CSF, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p40, IL-13, IL-15, IL-17, IL-21, KC, Leptin, LIX, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, PF-4, RANTES, TARC, TCA-3, TNF alpha, TNFRI, VEGF. | |
| Maus | |
| Kolorimetrisches Mikrotiterplatten-Lesegerät | |
| C5, C5a, He | |
| < 10 % | |
| HRP | |
| RUO | |
| Plasma, 1 μl; Serum, 1 μl; Überstand, 100 μl | |
| RP23-223C17.1, C5, C5a, He | |
| 1 Std 20 Min |